Part:BBa_K4294410
mNeonGreen Codon Optimised for E Coli
mNeonGreen is a green/yellow fluorescent protein which presents optimum absorbance at 506λ (nm) and optimum emission at 517λ (nm). It was reported as the brightest monomeric green or yellow fluorescent protein at the time. Actually, it is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs.
Usage and Biology
Fluorescent proteins are particularly useful in genetically encoded tags to visualise gene products and cellular compartments in living cells and organisms. In order to improve emitted signals and make them versatile tools, diverse fluorescent protein variants with different spectral and photophysical properties have been developed. The most famous among them is the Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, which revolutionised cell and developmental biology research. However, in 2013 Shaner and collaborators engineered the amphioxus Branchiostoma lanceolatum multimeric yellow fluorescence protein (LanYFP) to produce the monomeric mNeonGreen protein. It seems to be more stable and less sensitive to laser induced bleaching than EGFP. Therefore, mNeonGreen is particularly suitable for confocal and super resolution microscopy, especially when fusion proteins are investigated, which are expressed at low levels. It has already been expressed in multicellular organisms like C.elegans.
Figure 1: Excitation and Emission wavelengths of mNeonGreen
Sequence
Figure 2: Sequence comparison of mNeonGreen with EGFP
mNeonGreen consists of 237 amino acids, which translates into 26.6 kDa molecular weight. mNeonGreen is evolutionarily distant from jellyfish-derived fluorescent proteins. At sequence level, mNeonGreen shares just 20-25% sequence identity with common GFP derivatives.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] mNeonGreen, FPbase :: The Fluorescent Protein Database, https://www.fpbase.org/protein/mneongreen/ October 12 2022
[2] Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi, Jennifer Tsang, Addgene Blog October 12 2022
[3] Hostettler L, Grundy L, Käser-Pébernard S, Wicky C, Schafer WR, Glauser DA. The Bright Fluorescent Protein mNeonGreen Facilitates Protein Expression Analysis In Vivo. G3 (Bethesda). 2017 Feb 9;7(2):607-615. doi: 10.1534/g3.116.038133. PMID: 28108553; PMCID: PMC5295605.
[4] Shaner, N., Lambert, G., Chammas, A. et al. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods 10, 407–409 (2013). https://doi.org/10.1038/nmeth.2413
[5] mNeonGreen vs GFP, https://www.ptglab.com/news/blog/mneongreen-vs-gfp/?fbclid=IwAR25JJISJurn_cDT4b0aRc3z2fpFbfk1OEvFHOgRLqnHOtCcbQdh5MdD0sE, October 12 2022
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